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DSMZ

DSMZ 1376 . METHYLOCOCCUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1376 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 0.10 g 0.1 0.989
MgSO4 x 7 H2O 0.10 g 0.1 0.406
CaCl2 x 2 H2O 0.02 g 0.02 0.136
KBr 0.01 g 0.01 0.084
Trace Element Solution 0.10 ml - -
Vitamin solution 0.10 ml - -
Selenite-tungstate solution 0.10 ml - -
FeNa-EDTA 0.10 ml - -
Phosphate Buffer, pH 6.7 2.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the medium without the vitamin solution and the phosphate buffer. Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 50% methane to the gas phase and autoclave at 121°C for 15 minutes. Autoclave the phosphate buffer separately. When the growth medium is cool add 0.1 ml/l of vitamin solution (filter sterilized) and 2 ml/l of the phosphate buffer - if too warm the phosphate will precipitate. Liquid cultures should be grown with shaking.

Trace Element Solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.25 g 0.25 0.744
ZnSO4 x 7 H2O 0.40 g 0.4 1.391
MnCl2 x 4 H2O 0.02 g 0.02 0.101
H3BO3 0.02 g 0.02 0.323
Na2 MoO4 x 2 H2O 0.04 g 0.04 0.165
NiCl2 x 2 H2O 0.01 g 0.01 0.077
CuSO4 x 5 H2O 0.20 g 0.2 0.801
CoCl2 x 6 H2O 0.05 g 0.05 0.385
Distilled water 1000.00 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine-HCl x 2 H2O 10 mg 0.01 0.027
Nicotinic acid 20 mg 0.02 0.162
Pyridoxamine hydrochloride 10 mg 0.01 0.049
p-Aminobenzoic acid 10 mg 0.01 0.073
Riboflavin 20 mg 0.02 0.053
Biotin 1 mg 1.0e-3 0.004
Vitamin B12 5 mg 0.005 0.004
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Phosphate Buffer, pH 6.7 #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 37.425 g 37.425 275.012
Na2HPO4 48.950 g 48.95 344.818
Distilled water 1000.000 ml - -
1 pH 6.7

FeNa-EDTA #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeNa-EDTA 4.5 g 4.5 -
Distilled water 1000.0 ml - -

Phosphate Buffer, pH 8-9 #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 11.87 g - -
Distilled water 1000.00 ml - -
1 After the disodium phosphate has been dissolved in distilled water, pH should be between 8-9 without adjustment.
2 Autoclave at 121 °C for 15 min.

Metadata

Last modified: 02.06.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.7
Equipment needed: Autoclave
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Gas composition

  • 50 % CH4

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KNO3 0.1 0.989
MgSO4 x 7 H2O 0.1 0.406
Na2HPO4 0.098 0.69
KH2PO4 0.075 0.55
CaCl2 x 2 H2O 0.02 0.136
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