DSMZ 1378 . MO-CFXI MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.000 g 25 427.789
MgCl2 x 6 H2O 4.000 g 4 19.675
KCl 0.500 g 0.5 6.707
CaCl2 x 2 H2O 1.000 g 1 6.802
NH4Cl 0.535 g 0.535 10.002
KH2PO4 0.100 g 0.1 0.735
Tryptone 1.000 g 1 14.069
Glucose 1.800 g 1.8 9.991
Sodium pyruvate 0.550 g 0.55 4.998
Yeast extract 1.000 g 1 -
NaHCO3 2.000 g 2 23.808
Cysteine-HCl 0.300 g 0.3 1.903
Resazurin
(1mg/ml)
1.000 ml - -
Trace element solution 1.000 ml - -
Distilled water 1000.000 ml - -
Na2S 0.030 % 0.3 3.795
Vitamin solution 2.000 ml - -
1 pH 7.0
2 Prepare under N2/CO2 (80/20) gas.
3 Autoclave and add the following components after autoclaving from stock solutions:
4 sterile Na2S solution end concentration 0.03%
5 and filter-sterile Vitamin solution (see below) 0.2 ml / 100 ml.
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 1.27 g 1.27 6.388
ZnCl2 0.14 g 0.14 1.027
MnCl2 x 4 H2O 0.20 g 0.2 1.011
Na2SeO4 2.00 mg 0.002 0.011
H3BO3 6.00 mg 0.006 0.097
Na2WO4 x 2 H2O 3.00 mg 0.003 0.009
CuCl2 x 2 H2O 1.00 mg 1.0e-3 0.006
CoCl2 x 6 H2O 0.13 g 0.13 1.001
AlCl3 0.01 g 0.01 0.075
NiCl2 0.01 g 0.01 0.077
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.9 mg 0.005 0.02
Folic acid 8.8 mg 0.009 0.02
Pyridoxine hydrochloride 4.1 mg 0.004 0.02
Thiamine-HCl x 2 H2O 6.7 mg 0.007 0.018
Riboflavin 7.5 mg 0.008 0.02
Nicotinic acid 2.4 mg 0.002 0.019
D-Calcium pantothenate 9.5 mg 0.01 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 2.7 mg 0.003 0.02
Lipoic acid 4.1 mg 0.004 0.02
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 25 427.789
MgCl2 x 6 H2O 4 19.675
NaHCO3 2 23.808
Glucose 1.8 9.991
Tryptone 1 14.069
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain