Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
NaCl | 25.000 | g | ||
|
MgCl2 x 6 H2O | 4.000 | g | ||
|
KCl | 0.500 | g | ||
|
CaCl2 x 2 H2O | 1.000 | g | ||
|
NH4Cl | 0.535 | g | ||
|
KH2PO4 | 0.100 | g | ||
|
Tryptone | 1.000 | g | ||
|
Glucose | 1.800 | g | ||
|
Sodium pyruvate | 0.550 | g | ||
|
Yeast extract | 1.000 | g | ||
|
NaHCO3 | 2.000 | g | ||
|
Cysteine-HCl | 0.300 | g | ||
|
Resazurin
(1mg/ml) |
1.000 | ml | ||
|
Trace element solution | 1.000 | ml | ||
|
Distilled water | 1000.000 | ml | ||
|
Na2S | 0.030 | % | ||
|
Vitamin solution | 2.000 | ml | ||
1 pH 7.0 | |||||
2 Prepare under N2/CO2 (80/20) gas. | |||||
3 Autoclave and add the following components after autoclaving from stock solutions: | |||||
4 sterile Na2S solution end concentration 0.03% | |||||
5 and filter-sterile Vitamin solution (see below) 0.2 ml / 100 ml. |
Compound | Amount | Unit | |
---|---|---|---|
|
FeCl2 x 4 H2O | 1.27 | g |
|
ZnCl2 | 0.14 | g |
|
MnCl2 x 4 H2O | 0.20 | g |
|
Na2SeO4 | 2.00 | mg |
|
H3BO3 | 6.00 | mg |
|
Na2WO4 x 2 H2O | 3.00 | mg |
|
CuCl2 x 2 H2O | 1.00 | mg |
|
CoCl2 x 6 H2O | 0.13 | g |
|
AlCl3 | 0.01 | g |
|
NiCl2 | 0.01 | g |
|
Na2MoO4 x 2 H2O | 0.02 | g |
|
Distilled water | 1000.00 | ml |
Compound | Amount | Unit | |
---|---|---|---|
|
Biotin | 4.9 | mg |
|
Folic acid | 8.8 | mg |
|
Pyridoxine hydrochloride | 4.1 | mg |
|
Thiamine-HCl x 2 H2O | 6.7 | mg |
|
Riboflavin | 7.5 | mg |
|
Nicotinic acid | 2.4 | mg |
|
D-Calcium pantothenate | 9.5 | mg |
|
Vitamin B12 | 0.1 | mg |
|
p-Aminobenzoic acid | 2.7 | mg |
|
Lipoic acid | 4.1 | mg |
|
Distilled water | 1000.0 | ml |