1381 .
½ S° HELIORESTIS MEDIUM
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Equipment needed:
Final pH:
9.0 - 9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA
(1%) |
1.000 | ml | ||
|
|
KH2PO4 | 0.500 | g | ||
|
|
NH4Cl | 0.500 | g | ||
|
|
Bicine | 1.630 | g | ||
|
|
MgSO4 x 7 H2O | 0.200 | g | ||
|
|
CaCl2 | 0.075 | g | ||
|
|
Na2CO3 | 2.500 | g | ||
|
|
NaHCO3 | 2.500 | g | ||
|
|
Na-acetate | 1.000 | g | ||
|
|
Yeast extract | 0.100 | g | ||
|
|
Na2S x 9 H2O | 0.250 | g | ||
|
|
Na-pyruvate | 1.000 | g | ||
|
|
Trace elements | 1.000 | ml | ||
|
|
Vitamin B12 | 20.000 | µg | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 Prepare under N2 gas and add vitamin B12, NaHCO3, Na2CO3 and sulfide as single sterile stock solutions to sterile medium after autoclaving. Adjust pH to 9 - 9.5. | |||||
Trace elements #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
EDTA | 5.2 | g |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
MnCl2 x 4 H2O | 0.1 | g |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
H3BO3 | 6.0 | mg |
|
|
CuCl2 x 2 H2O | 17.0 | mg |
|
|
Na2MoO4 x 2 H2O | 188.0 | mg |
|
|
NiCl2 x 6 H2O | 25.0 | mg |
|
|
ZnCl2 | 70.0 | mg |
|
|
VOSO4 x 2 H2O | 30.0 | mg |
|
|
Na2WO4 x 2 H2O | 2.0 | mg |
|
|
NaHSeO3 | 2.0 | mg |
|
|
Deionized water | 1000.0 | ml |