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DSMZ

DSMZ 1385 . NITROLANCETUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1385 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.15 g 0.15 1.102
Trace elements solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the liquid medium. Adjust to pH 7.2. Dispense the medium into serum bottles. Inject 10 ml CO2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
CaCl2 x 2 H2O 50.00 mg 0.05 0.34
MgSO4 x 7 H2O 260.00 mg 0.26 1.055
NH4HCO3 395.00 mg 0.395 -
KNO2 4.25 g 4.25 -
Sodium formate 2.72 g 2.72 39.996

Trace elements solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 6 H2O 2.20 g 2.2 14.482
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust to pH 3.0-4.0

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.2
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KNO2 4.25 -
Sodium formate 2.72 39.996
NH4HCO3 0.395 -
MgSO4 x 7 H2O 0.26 1.055
KH2PO4 0.15 1.102
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Cultivation metadata from 0 strains BacDive

phyla 1 1 strain