Name: LAMPROBACTER MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1389 #

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.50	g	0.5	3.674
NaCl	40.00	g	40	684.463
NH₄Cl	0.50	g	0.5	9.348
MgCl₂ x 6 H₂O	0.20	g	0.2	0.984
NaHCO₃	1.50	g	1.5	17.856
KCl	0.33	g	0.33	4.427
Na₂S x 9 H₂O	0.50	g	0.5	2.082
Na₂S₂O₃ x 5 H₂O	0.50	g	0.5	2.015
Na-acetate	0.50	g	0.5	6.095
Yeast extract	0.50	g	0.5	-
Trace elements solution (Pfennig & Lippert,1966)	1.00	ml	-	-
Vitamin B₁₂	10.00	µg	1.0e-5	7.4e-6
Distilled water	1000.00	ml	-	-
1 Adjust pH to 7.5.
2 Prepare the medium without the NaHCO₃, Na₂S x 9 H₂O, Na₂S2O3 x 5 H₂O and vitamin B₁₂, under a nitrogen atmosphere. Autoclave and add the vitamin B₁₂ from a filter-sterilised stock solution and the NaHCO₃, Na₂S x 9 H₂O, Na₂S2O3 x 5 H₂O from sterile stock solutions. The final pH of the medium should be 7.5.
3 For Allochromatium phaeobacterium DSM 19781 reduce the amount of NaCl to 10.0 g/l and increase the amount of NH₄Cl to 0.64 g/l.

Trace elements solution (Pfennig & Lippert,1966) #

1000

Solution occurs in 28 other media (first defined in Medium 1369).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA	5.00	g	5	17.109
FeSO₄ x 7 H₂O	2.20	g	2.2	7.913
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
MnCl₂ x 4 H₂O	0.03	g	0.03	0.152
H₃BO₃	0.03	g	0.03	0.485
CoCl₂ x 6 H₂O	0.20	g	0.2	1.54
CuCl₂ x 2 H₂O	0.03	g	0.03	0.176
NiCl₂ x 6 H₂O	0.03	g	0.03	0.231
Na₂MoO₄ x 2 H₂O	0.03	g	0.03	0.124
Distilled water	1000.00	ml	-	-
1 pH 3.0-4.0

All strains for this medium:	1
Strains with modifications:	1

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