139
139 .
HATHEWAYA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 139 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase soy broth
(BD BBL) |
15.00 | g | 15 | - | |
|
Proteose peptone
(BD BBL) |
50.00 | g | 50 | - | |
| Na2HPO4 | 9.00 | g | 9 | 63.399 | |
| KH2PO4 | 1.92 | g | 1.92 | 14.109 | |
| MgSO4 x 7 H2O | 0.08 | g | 0.08 | 0.325 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients, adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Proteose peptone | 50 | - |
| Trypticase soy broth | 15 | - |
| Na2HPO4 | 9 | 63.399 |
| KH2PO4 | 1.92 | 14.109 |
| MgSO4 x 7 H2O | 0.08 | 0.325 |
| Show more | ||
