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1391. MMJHSY MEDIUM MODIFIED

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 19.901 340.514
2 0.09 g 0.09 0.514
3 0.07 g 0.07 0.512
4 0.80 g 0.796 5.415
5 1.00 g 0.995 18.602
6 1.00 g 0.995 11.707
7 4.00 g 3.98 16.148
8 3.00 g 2.985 14.683
9 0.33 g 0.328 4.404
10 0.01 g 0.01 0.036
11 2.00 g 1.99 8.018
12 0.01 g 0.01 0.041
13 2.00 g 1.99 -
14 Trace mineral solution 5.00 ml - -
15 1000.00 ml 993.241 55133.2
16 Vitamin solution
(final concentration 1.0 ml/l)
1.00 ml - -
17
(final concentration 0.1 %)
0.10 % 1 11.904
Instructions:
  1. Prepare the medium under N2/CO2 (80%N2, 20%CO2) and fill in , the pH of the medium is adjusted with NaOH to 6.8. After autoclaving, the separately autoclaved, concentrated solutions (#16-17) including each of follows are added to the medium. Then a mix gas (80%H2, 20%CO2) is purged for 5 min.
  2. Finally, the mix gas (77%H2, 20%CO2, 3%O2) is compressed into gas phase (>80% volume of the tube or bottle) at 2 atm.
Solution occurs in 1 other media (first defined in medium 1121 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.500 g 1.5 7.848
2 0.500 g 0.5 2.958
3 0.500 g 0.5 1.779
4 0.180 g 0.18 0.626
5 0.010 g 0.01 0.04
6 0.020 g 0.02 0.042
7 0.010 g 0.01 0.162
8 0.001 g 1.0e-3 0.004
9 0.010 g 0.01 0.038
10 0.010 g 0.01 0.097
11 0.010 g 0.01 0.039
12 0.100 g 0.1 0.421
13 0.100 g 0.1 0.38
14 1.000 g 1 -
15 0.100 g 0.1 0.4
16 1000.000 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1

Gas composition

  • 20 % CO2
  • 77 % H2
  • 3 % O2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 999.2 55464
NaCl 19.901 340.514
MgSO4 x 7 H2O 3.98 16.148
MgCl2 x 6 H2O 2.985 14.683
Na2S2O3 x 5 H2O 1.99 8.018
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain

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