1391
1391 .
MMJHSY MEDIUM (modified)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1391 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 20 | 342.231 | |
| K2HPO4 | 0.09 | g | 0.09 | 0.517 | |
| KH2PO4 | 0.07 | g | 0.07 | 0.514 | |
| CaCl2 x 2 H2O | 0.80 | g | 0.8 | 5.442 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| NaNO3 | 1.00 | g | 1 | 11.765 | |
| MgSO4 x 7 H2O | 4.00 | g | 4 | 16.229 | |
| MgCl2 x 6 H2O | 3.00 | g | 3 | 14.756 | |
| KCl | 0.33 | g | 0.33 | 4.427 | |
| FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.036 | |
| Na2S2O3 x 5 H2O | 2.00 | g | 2 | 8.058 | |
| Fe(III) citrate | 0.01 | g | 0.01 | 0.041 | |
| Yeast extract | 2.00 | g | 2 | - | |
| Trace mineral solution | 5.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium under N2/CO2 (80%N2, 20%CO2) and fill in , the pH of the medium is adjusted with NaOH to 6.8. After autoclaving, the separately autoclaved, concentrated solutions including each of follows are added to the medium. Then a mix gas (80%H2, 20%CO2) is purged for 5 min. | |||||
| 2 Finally, the mix gas (77%H2, 20%CO2, 3%O2) is compressed into gas phase (>80% volume of the tube or bottle) at 2 atm. | |||||
| Wolin's vitamin solution | 1.00 | ml | - | - | |
| NaHCO3 | 0.10 | % | 1 | 11.904 | |
Solution occurs in 1
other media (first defined in Medium 1121).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - |
| CoSO4 x 7 H2O | 0.50 | g | 0.5 | 3.226 |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 |
| H3BO3 | 0.01 | g | 0.01 | 0.162 |
| Na2MoO4 x 2 H2O | 1.00 | mg | 1.0e-3 | 0.004 |
| SrCl2 x 6 H2O | 0.01 | g | 0.01 | 0.038 |
| NaBr | 0.01 | g | 0.01 | 0.097 |
| KI | 0.01 | g | 0.01 | 0.039 |
| NiCl2 x 6 H2O | 0.10 | g | 0.1 | 0.772 |
| Na2SeO3 x 5 H2O | 0.10 | g | 0.1 | 0.38 |
| Fe2(SO4)3 x n H2O | 1.00 | g | 1 | 2.501 |
| H2WO4 | 0.10 | g | 0.1 | 0.4 |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 77 % H2
- 20 % CO2
- 3 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 20 | 342.231 |
| MgSO4 x 7 H2O | 4 | 16.229 |
| MgCl2 x 6 H2O | 3 | 14.756 |
| Na2S2O3 x 5 H2O | 2 | 8.058 |
| Yeast extract | 2 | - |
| Show more | ||
