DSMZ 13 . BACILLUS ACIDOCALDARIUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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* Modified for Alicyclobacillus fastidiosus DSM 17978

Plus MnSO4 (see medium 1); pH: 4.2

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 500 ml - -
1 Sterilize solution A and B separately at 121°C for 15 min. Cool to 50°C and then combine.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.00 g 2 -
(NH4)2SO4 0.20 g 0.4 3.027
MgSO4 x 7 H2O 0.50 g 1 4.057
CaCl2 x 2 H2O 0.25 g 0.5 3.401
KH2PO4 0.60 g 1.2 8.818
Distilled water 500.00 ml - -
1 Adjust pH to 3.0 - 4.0.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 1 g 2 11.101
Agar 20 g 40 118.931
Distilled water 500 ml - -

Metadata

Last modified: 04.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 4.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 8
Strains with modifications: 7
Alicyclobacillus fastidiosus DSM 17978

  4.2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.466
Glucose 1 5.551
Yeast extract 1 -
KH2PO4 0.6 4.409
MgSO4 x 7 H2O 0.5 2.029
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Cultivation metadata from 8 strains BacDive

pH 4 - 6 6 strains
phyla 1 8 strains
oxygen aerobe 6 strains
temperature thermophilic 8 strains