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DSMZ

DSMZ 1406 . HALARSENATIBACTER MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1406 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 151.000 g 151 2583.85
Na2SO4 84.000 g 84 591.383
K2SO4 25.000 g 25 143.464
(NH4)2SO4 0.050 g 0.05 0.378
KH2PO4 0.080 g 0.08 0.588
K2HPO4 0.150 g 0.15 0.861
MgSO4 x 7 H2O 0.025 g 0.025 0.101
Na2WO4 0.075 g 0.075 0.255
H3BO3 4.000 g 4 64.683
Na2SeO4 0.010 mg 1.0e-5 5.3e-5
Trace Element Solution SL10 3.000 ml - -
Na2CO3 36.000 g 36 339.661
Distilled water 1000.000 ml - -
1 Prepare the liquid medium without the following. NaHCO3, yeast extract, Na-lactate, arsenate. Adjust to pH 9.5. Boil the medium in a water bath and cool under N2. to room temperature. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
NaHCO3 , after autoclaving 6.000 g 6 71.423
Yeast extract , after autoclaving 1.000 g 1 -
Na-lactate , after autoclaving 10.000 mM 1.121 10
Arsenate , after autoclaving 5.000 mM 0.695 5
Cysteine HCl , after autoclaving 0.025 % 0.25 2.063
Vitamin solution , after autoclaving 10.000 ml - -

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace Element Solution SL10 #

1000
Equipment needed: Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		7.700 ml 1.925 52.798
FeSO4 x 7 H2O 1.500 g 1.5 5.395
ZnCl2 0.070 g 0.07 0.514
MnCl2 x 4 H2O 0.100 g 0.1 0.505
H3BO3 0.300 g 0.3 4.851
CoCl2 x 6 H2O 0.190 g 0.19 1.463
CuCl2 x 2 H2O 2.000 mg 0.002 0.012
NiCl2 x 6 H2O 0.024 g 0.024 0.185
Na2MoO4 x 2 H2O 0.036 g 0.036 0.149
Distilled water 1000.000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5
Equipment needed: Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 151 2583.85
Na2SO4 84 591.383
Na2CO3 36 339.661
K2SO4 25 143.464
NaHCO3 6 71.423
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain