1416
1416 .
MST-MEDIUM, AUTOTROPHIC GROWTH
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1416 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2S2O3 x 5 H2O | 5.0 | g | 5 | 20.146 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| MgSO4 x 7 H2O | 0.5 | g | 0.5 | 2.029 | |
| K2HPO4 | 4.0 | g | 4 | 22.965 | |
| KH2PO4 | 1.5 | g | 1.5 | 11.023 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare separate solutions of sodium thiosulfate and magnesium sulfate. Adjust solutions to pH 7.5 -8.0 and sterilize by autoclaving. Add sterile trace element solution after autoclving and combining the solutions. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.5 - 8.0 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2S2O3 x 5 H2O | 5 | 20.146 |
| K2HPO4 | 4 | 22.965 |
| KH2PO4 | 1.5 | 11.023 |
| NH4Cl | 1 | 18.695 |
| MgSO4 x 7 H2O | 0.5 | 2.029 |
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