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DSMZ

DSMZ 1419 . HYPHOMICROBIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1419 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral Salts Solution A 50.0 ml - -
Mineral Salts Solution B 1000.0 ml - -
1 Autoclave solution A and B separately and combine after cooling.
2 Prepare the liquid medium. Before inoculation add from sterile stock solutions:
Vitamin solution 10.0 ml - -
Vitamin B12
(0.1%) 		2.5 µl 2.5e-6 1.8e-6
3 For solid media add 15.0 g agar to solution B

Mineral Salts Solution A #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.36 g 27.2 199.875
Na2HPO4 x 2 H2O 2.68 g 53.6 377.574
Methylamine hydrochloride 3.38 g 67.6 1001.18
Distilled water 50.00 ml - -
1 Adjust pH to 7.5

Mineral Salts Solution B #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.50 g 0.5 3.784
MgSO4 x 7 H2O 0.20 g 0.2 0.811
Bacto peptone 0.25 g 0.25 -
Trace Element Solution 2.00 ml - -
Hutner's salts 20.00 ml - -
Vitamin solution , add after autoclaving 10.00 ml - -
Vitamin B12 , add after autoclaving
(0.1%) 		2.50 µl 2.5e-6 1.8e-6
Distilled water 1000.00 ml - -
Agar , for solid medium 15.00 g 15 44.599
1 Adjust pH to 7.5

Trace Element Solution #

100
Solution occurs in 1 other media (first defined in Medium 1419).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.199 g 1.99 13.536
FeSO4 x 7 H2O 0.100 g 1 3.597
MnSO4 x H2O 0.035 g 0.35 2.071
Na2MoO4 x 2 H2O 0.050 g 0.5 2.067
Distilled water 100.000 ml - -

Hutner's salts #

1000
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 0.007
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid.
2 Adjust pH to 7.0 with KOH (~7.3 g).
3 Dissolve other salts separately and combine.
4 Adjust pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Vitamin solution #

1010
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.004
Pyridoxine hydrochloride 10.0 mg 0.01 0.048
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.04
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 9.9e-5 7.3e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Distilled water 1000.0 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 14.286 42.475
Methylamine hydrochloride 3.219 47.675
Na2HPO4 x 2 H2O 2.552 17.98
KH2PO4 1.295 9.518
MgSO4 x 7 H2O 0.566 2.295
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