DSMZ 1419 . HYPHOMICROBIUM MEDIUM

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Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral Salts Solution A 50.0 ml - -
Mineral Salts Solution B 1000.0 ml - -
1 Autoclave solution A and B separately and combine after cooling.
2 Prepare the liquid medium. Before inoculation add from sterile stock solutions:
Vitamin solution 10.0 ml - -
Vitamin B12
(0.1%)
2.5 µl 2.5e-6 1.8e-6
3 For solid media add 15.0 g agar to solution B
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.36 g 27.2 199.875
Na2HPO4 x 2 H2O 2.68 g 53.6 377.574
Methylamine hydrochloride 3.38 g 67.6 1001.18
Distilled water 50.00 ml - -
1 Adjust pH to 7.5
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.50 g 0.5 3.784
MgSO4 x 7 H2O 0.20 g 0.2 0.811
Bacto peptone 0.25 g 0.25 -
Trace Element Solution 2.00 ml - -
Hutner's salts 20.00 ml - -
Vitamin solution , add after autoclaving 10.00 ml - -
Vitamin B12 , add after autoclaving
(0.1%)
2.50 µl 2.5e-6 1.8e-6
Distilled water 1000.00 ml - -
Agar , for solid medium 15.00 g 15 44.599
1 Adjust pH to 7.5
Solution occurs in 1 other media (first defined in Medium 1419).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.199 g 1.99 13.536
FeSO4 x 7 H2O 0.100 g 1 3.597
MnSO4 x H2O 0.035 g 0.35 2.071
Na2MoO4 x 2 H2O 0.050 g 0.5 2.067
Distilled water 100.000 ml - -
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
Metall salt sol. 44 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
Solution occurs in 18 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.004
Pyridoxine hydrochloride 10.0 mg 0.01 0.048
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.04
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 9.9e-5 7.3e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Distilled water 1000.0 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 14.286 42.475
Methylamine hydrochloride 3.219 47.675
Na2HPO4 x 2 H2O 2.552 17.98
KH2PO4 1.295 9.518
MgSO4 x 7 H2O 0.566 2.295
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