Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Mineral Salts Solution A | 50.0 | ml | ||
|
Mineral Salts Solution B | 1000.0 | ml | ||
1 Autoclave solution A and B separately and combine after cooling. | |||||
2 Prepare the liquid medium. Before inoculation add from sterile stock solutions: | |||||
|
Vitamin solution | 10.0 | ml | ||
|
Vitamin B12
(0.1%) |
2.5 | µl | ||
3 For solid media add 15.0 g agar to solution B |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
KH2PO4 | 1.36 | g | ||
|
Na2HPO4 x 2 H2O | 2.68 | g | ||
|
Methylamine hydrochloride | 3.38 | g | ||
|
Distilled water | 50.00 | ml | ||
1 Adjust pH to 7.5 |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
(NH4)2SO4 | 0.50 | g | ||
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
Bacto peptone | 0.25 | g | ||
|
Trace Element Solution | 2.00 | ml | ||
|
Hutner's salts | 20.00 | ml | ||
|
Vitamin solution , add after autoclaving | 10.00 | ml | ||
|
Vitamin B12
, add after autoclaving
(0.1%) |
2.50 | µl | ||
|
Distilled water | 1000.00 | ml | ||
|
Agar , for solid medium | 15.00 | g | ||
1 Adjust pH to 7.5 |
Compound | Amount | Unit | |
---|---|---|---|
|
CaCl2 x 2 H2O | 0.199 | g |
|
FeSO4 x 7 H2O | 0.100 | g |
|
MnSO4 x H2O | 0.035 | g |
|
Na2MoO4 x 2 H2O | 0.050 | g |
|
Distilled water | 100.000 | ml |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | ||
|
MgSO4 x 7 H2O | 29.700 | g | ||
|
CaCl2 x 2 H2O | 3.335 | g | ||
|
(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | ||
|
FeSO4 x 7 H2O | 99.000 | mg | ||
|
"Metals 44" | 50.000 | ml | ||
|
Distilled water | 950.000 | ml | ||
1 Dissolve the nitrilotriacetic acid. | |||||
2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
3 Dissolve other salts separately and combine. | |||||
4 Adjust pH to 6.8 with NaOH or H2SO4. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Na-EDTA | 250.0 | mg | ||
|
ZnSO4 x 7 H2O | 1095.0 | mg | ||
|
FeSO4 x 7 H2O | 500.0 | mg | ||
|
MnSO4 x H2O | 154.0 | mg | ||
|
CuSO4 x 5 H2O | 39.2 | mg | ||
|
Co(NO3)2 x 6 H2O | 24.8 | mg | ||
|
Na2B4O7 x 10 H2O | 17.7 | mg | ||
|
Distilled water | 1000.0 | ml | ||
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. |
Compound | Amount | Unit | |
---|---|---|---|
|
Biotin | 2.0 | mg |
|
Folic acid | 2.0 | mg |
|
Pyridoxine hydrochloride | 10.0 | mg |
|
Thiamine-HCl x 2 H2O | 5.0 | mg |
|
Riboflavin | 5.0 | mg |
|
Nicotinic acid | 5.0 | mg |
|
D-Calcium pantothenate | 5.0 | mg |
|
Vitamin B12 | 0.1 | mg |
|
p-Aminobenzoic acid | 5.0 | mg |
|
Distilled water | 1000.0 | ml |