1425 .
SSE/HD 1:10 MEDIUM (HEPES)
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Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 0.50 | g | ||
|
|
Yeast extract | 0.25 | g | ||
|
|
Glucose | 0.10 | g | ||
|
|
HEPES
(10 mM final concentration) |
1.95 | g | ||
|
|
SSE (double concentrated) | 500.00 | ml | ||
|
|
Trace element solution SL-10 | 1.00 | ml | ||
|
|
Distilled water | 500.00 | ml | ||
| 1 Add to 1000 ml of medium after autoclaving: | |||||
|
|
Vitamin solution | 1.00 | ml | ||
| 2 adjust pH to 7.0 | |||||
SSE (double concentrated) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
CaCl2 x 2 H2O | 0.2938 | g | ||
|
|
NH4Cl | 0.1069 | g | ||
|
|
MgCl2 x 6 H2O | 0.2036 | g | ||
|
|
(NH4)2SO4 | 0.1983 | g | ||
|
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MgSO4 x 7 H2O | 0.7390 | g | ||
|
|
CaSO4 x 2 H2O | 0.8606 | g | ||
|
|
Ca(NO3)2 x 4 H2O | 0.2360 | g | ||
|
|
Na NO3 | 0.4240 | g | ||
|
|
KH2PO4 | 0.0700 | g | ||
|
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FeSO4 x 7 H2O | 0.0111 | g | ||
|
|
K2SO4 | 0.0870 | g | ||
|
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Distilled water | 1000.0000 | ml | ||
| 1 Dissolve by shaking overnight. Sterilize by autoclaving. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine-HCl x 2 H2O | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
D-Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |