DSMZ 142a . THIOMICROSPIRA PSYCHROPHILA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 15.00 g 15 256.674
(NH4)2SO4 1.00 g 1 7.568
MgSO4 x 7 H2O 1.50 g 1.5 6.086
CaCl2 x 2 H2O 0.42 g 0.42 2.857
Trace element solution SL-10 1.00 ml - -
Bromothymol blue
(0.1% w/v)
4.00 ml 0.004 0.006
K2HPO4 0.50 g 0.5 2.871
Na2S2O3 x 5 H2O 5.00 g 5 20.146
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except hydrogenphosphate, thiosulfate and vitamins), adjust pH to 6.0 and autoclave. Phosphate and thiosulfate are autoclaved separately each in 5% of the final volume. The vitamin solution is sterilized by filtration. Adjust pH of the complete medium to 7.6 with a sterile 5% (w/v) Na2CO3 solution.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.6
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 4
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
Na2S2O3 x 5 H2O 5 20.146
MgSO4 x 7 H2O 1.5 6.086
(NH4)2SO4 1 7.568
K2HPO4 0.5 2.871
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Cultivation metadata from 4 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 2 strains
phyla 2 4 strains
nutrition chemolithoautotroph 2 strains
oxygen aerobe 2 strains
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