142a .
THIOMICRORHABDUS MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 15.00 | g | ||
|
|
(NH4)2SO4 | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.42 | g | ||
|
|
Trace element solution SL-10 | 1.00 | ml | ||
|
|
Bromothymol blue
(0.1% w/v) |
4.00 | ml | ||
|
|
K2HPO4 | 0.50 | g | ||
|
|
Na2S2O3 x 5 H2O | 5.00 | g | ||
|
|
Seven vitamins solution | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except hydrogenphosphate, thiosulfate and vitamins), adjust pH to 6.0 and autoclave. Phosphate and thiosulfate are autoclaved separately each in 5% of the final volume. The vitamin solution is sterilized by filtration. Adjust pH of the complete medium to 7.6 with a sterile 5% (w/v) Na2CO3 solution. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Seven vitamins solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 100 | mg |
|
|
p-Aminobenzoic acid | 80 | mg |
|
|
D-(+)-biotin | 20 | mg |
|
|
Nicotinic acid | 200 | mg |
|
|
Calcium pantothenate | 100 | mg |
|
|
Pyridoxine hydrochloride | 300 | mg |
|
|
Thiamine-HCl x 2 H2O | 200 | mg |
|
|
Distilled water | 1000 | ml |