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1431. ALCALOPHILIC HALOPHILE MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.00 g 333.333 -
2 7.50 g 250 -
3 3.00 g 100 387.493
4 2.00 g 66.667 894.242
5 1.00 g 33.333 135.24
6 0.36 mg 0.012 0.061
7 50.00 mg 1.667 5.995
8 200.00 g 6666.67 114072
9 50.00 g 1666.67 15725.1
10 , might need strain adjustment 2.00 % - -
11 , might need strain adjustment
(10 %)
30.00 ml - -
Instructions:
  1. Liquid medium may be prepared by adding all components together. Agar (2%) medium should be prepared without Na2CO3 and sterilised by autoclaving. Add 20-30 ml of a sterile 10% Na2CO3 solution to the autoclaved medium containing agar so that the final pH is 9.0.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 6666.67 114072
Na2CO3 1666.67 15725.1
Yeast extract 333.333 -
Casamino Acid 250 -
Na3-citrate 100 387.493
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

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