1432
1432 .
ENHYDROBACTER MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1432 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Mineral salt solution
("Hutner/Cohen-Bazire") |
20.00 | ml | - | - | |
|
Casamino acids
(BD 223050) |
1.00 | g | 1 | - | |
| Glucose | 1.00 | g | 1 | 5.551 | |
|
Agar
(Bacto) |
15.00 | g | 15 | 44.599 | |
| Distilled water | 980.00 | ml | - | - | |
| 1 Sterilize 20 min. /121°C. After cooling to 60°C add to the medium: | |||||
| Biotin | 0.02 | mg | 2.0e-5 | 8.2e-5 | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.00 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.70 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.34 | g | 3.34 | 22.719 | |
| Na2MoO4 x 2 H2O | 12.67 | mg | 0.013 | 0.052 | |
| FeSO4 x 7 H2O | 99.00 | mg | 0.099 | 0.356 | |
| "Metals 44" | 50.00 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water. | |||||
Solution occurs in 34
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
| ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
| FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
| MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
| CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
| Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
| Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 1 | 5.551 |
| Casamino acids | 1 | - |
| MgSO4 x 7 H2O | 0.594 | 2.41 |
| Nitrilotriacetic acid | 0.2 | 1.046 |
| Show more | ||
