1433
1433 .
ACIDO-THERMOPHILE MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Acidothermus cellulolyticus DSM 8971
This strain uses the standard medium without modifications.
Growth conditions: 55 °C
Main sol. 1433 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| Na2HPO4 x 7 H2O | 0.10 | g | 0.1 | 0.704 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 0.02 | ml | - | - | |
|
Yeast extract
(Oxoid) |
1.00 | g | 1 | - | |
|
Sigmacell alpha Type 50
(Sigma S5504) |
5.00 | g | 5 | - | |
| Cellobiose | 0.50 | g | 0.5 | 1.461 | |
| Agar | 20.00 | g | 20 | 59.465 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust to pH 5.2 with H3PO4 prior to addition of carbon sources. | |||||
| 2 Autoclave at 121°C for 15 minutes. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Acidothermus cellulolyticus DSM 8971
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | 59.465 |
| Sigmacell alpha Type 50 | 5 | - |
| NH4Cl | 1 | 18.695 |
| KH2PO4 | 1 | 7.348 |
| Yeast extract | 1 | - |
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