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DSMZ

DSMZ 1440 . PIRELLULA MEDIUM (M31)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1440 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 950 ml - -
Solution 2 50 ml - -
1 After autoclaving add solution 2 to solution 1.

Solution 1 #

950
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.1 g 0.105 0.716
MgCl2 x 6 H2O 0.1 g 0.105 0.518
Hutner's salts 20.0 ml - -
Tris-HCl buffer
(0.1M, pH 7.5) 		50.0 ml - 5
Agar 18.0 g 18.947 56.336
Distilled water 880.0 ml - -
1 Adjust pH to 7.5, autoclave at 121°C for 20 min.

Solution 2 #

50
Solution occurs in 1 other media (first defined in Medium 1438).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
N-Acetylglucosamine 2.0 g 40 180.824
Na2HPO4 x 2 H2O 0.1 g 2 14.089
Vitamin solution 10.0 ml - -
Distilled water 40.0 ml - -
1 Adjust pH to 7.5, filter-sterilize

Vitamin solution #

1000
Solution occurs in 7 other media (first defined in Medium 1436).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
Biotin 4.0 mg 0.004 0.016
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Calcium pantothenate 10.0 mg 0.01 0.021
Folic acid 4.0 mg 0.004 0.009
Riboflavin 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Distilled water 1000.0 ml - -
1 filter-sterilized

Hutner's salts #

1000
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 0.007
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid.
2 Adjust pH to 7.0 with KOH (~7.3 g).
3 Dissolve other salts separately and combine.
4 Adjust pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 18 53.519
N-Acetylglucosamine 2 9.041
MgSO4 x 7 H2O 0.594 2.41
Nitrilotriacetic acid 0.2 1.046
Na2HPO4 x 2 H2O 0.1 0.704
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