1442
1442 .
PIRELLULA MEDIUM (M33a)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1442 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Gelatine | 2.00 | g | 2 | - | |
| Yeast extract | 0.25 | g | 0.25 | - | |
| Glucose | 0.25 | g | 0.25 | 1.388 | |
| Hutner's salts | 20.00 | ml | - | - | |
| Vitamin solution | 10.00 | ml | - | - | |
|
Tris-HCl buffer
(0.1M, bei Agar pH 8,5, pH 7.5) |
50.00 | ml | - | 5 | |
| Artifical sea water | 250.00 | ml | - | - | |
| Agar | 18.00 | g | 18 | 53.519 | |
| Distilled water | 670.00 | ml | - | - | |
| 1 Adjust pH to 7.5, autoclave at 121°C for 20 min. | |||||
| 2 For agar plates increase the pH of the Tris/HCl buffer to 8.5. | |||||
Solution occurs in 7
other media (first defined in Medium 1436).
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin B12 | 0.2 | mg | 2.0e-4 | 1.5e-4 | |
| Biotin | 4.0 | mg | 0.004 | 0.016 | |
| Thiamine-HCl x 2 H2O | 10.0 | mg | 0.01 | 0.027 | |
| Calcium pantothenate | 10.0 | mg | 0.01 | 0.021 | |
| Folic acid | 4.0 | mg | 0.004 | 0.009 | |
| Riboflavin | 10.0 | mg | 0.01 | 0.027 | |
| Nicotinamide | 10.0 | mg | 0.01 | 0.082 | |
| p-Aminobenzoic acid | 10.0 | mg | 0.01 | 0.073 | |
| Pyridoxine hydrochloride | 20.0 | mg | 0.02 | 0.097 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 filter-sterilized | |||||
Solution occurs in 1
other media (first defined in Medium 1442).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 23.47 | g | 23.47 | 401.608 |
| Na2SO4 | 3.92 | g | 3.92 | 27.598 |
| MgCl2 x 6 H2O | 4.98 | g | 4.98 | 24.496 |
| CaCl2 | 1.10 | g | 1.1 | 9.912 |
| NaHCO3 | 192.00 | mg | 0.192 | 2.286 |
| KCl | 664.00 | mg | 0.664 | 8.907 |
| KBr | 96.00 | mg | 0.096 | 0.807 |
| H3BO3 | 26.00 | mg | 0.026 | 0.42 |
| SrCl2 | 24.00 | mg | 0.024 | 0.151 |
| NaF | 3.00 | mg | 0.003 | 0.071 |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 24
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
| (NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | 0.007 | |
| FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
| "Metals 44" | 50.000 | ml | - | - | |
| Distilled water | 950.000 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid. | |||||
| 2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
| 3 Dissolve other salts separately and combine. | |||||
| 4 Adjust pH to 6.8 with NaOH or H2SO4. | |||||
Solution occurs in 34
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
| ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
| FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
| MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
| CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
| Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
| Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 7.5 - 8.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |