This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Solution 1 | 950 | ml | - | - |
Solution 2 | 50 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Hutner's salts | 20 | ml | - | - | |
Tris-HCl buffer
(0.1M, pH 7.5) |
50 | ml | - | 5 | |
Artifical sea water | 500 | ml | - | - | |
Agar | 18 | g | 18.947 | 56.336 | |
Distilled water | 380 | ml | - | - | |
1 Adjust pH to 7.5, autoclave at 121°C for 20 min. | |||||
2 For agar plates increase the pH of the Tris/HCl buffer to 8.5. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
N-Acetylglucosamine | 2.0 | g | 40 | 180.824 | |
Na2HPO4 x 2 H2O | 0.1 | g | 2 | 14.089 | |
Vitamin solution (double conc.) | 10.0 | ml | - | - | |
Distilled water | 40.0 | ml | - | - | |
1 Adjust pH to 7.5, filter-sterilize, and add to solution 1. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | 0.007 | |
FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
"Metals 44" | 50.000 | ml | - | - | |
Distilled water | 950.000 | ml | - | - | |
1 Dissolve the nitrilotriacetic acid. | |||||
2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
3 Dissolve other salts separately and combine. | |||||
4 Adjust pH to 6.8 with NaOH or H2SO4. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 23.47 | g | 23.47 | 401.608 |
Na2SO4 | 3.92 | g | 3.92 | 27.598 |
MgCl2 x 6 H2O | 10.64 | g | 10.64 | 52.336 |
CaCl2 | 1.10 | g | 1.1 | 9.912 |
NaHCO3 | 192.00 | mg | 0.192 | 2.286 |
KCl | 664.00 | mg | 0.664 | 8.907 |
KBr | 96.00 | mg | 0.096 | 0.807 |
H3BO3 | 26.00 | mg | 0.026 | 0.42 |
SrCl2 | 24.00 | mg | 0.024 | 0.151 |
NaF | 3.00 | mg | 0.003 | 0.071 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Biotin | 4.0 | mg | 0.004 | 0.016 | |
Folic acid | 4.0 | mg | 0.004 | 0.009 | |
Pyridoxine hydrochloride | 20.0 | mg | 0.02 | 0.097 | |
Riboflavine | 10.0 | mg | 0.01 | 0.027 | |
Thiamine-HCl x 2 H2O | 10.0 | mg | 0.01 | 0.027 | |
Nicotinamide | 10.0 | mg | 0.01 | 0.082 | |
D-Calcium pantothenate | 10.0 | mg | 0.01 | - | |
Vitamin B12 | 0.2 | mg | 2.0e-4 | 1.5e-4 | |
p-Aminobenzoic acid | 10.0 | mg | 0.01 | 0.073 | |
Distilled water | 1000.0 | ml | - | - | |
1 Store in the dark and cold (5°C). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Medium type: | Defined medium |
Final pH: | 7.5 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |