1446
1446 .
ALKALISPIRILLUM MOBILE MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1446 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 20 | 342.231 | |
| Na2S2O3 | 1.00 | g | 1 | 2.334 | |
| KH2PO4 | 0.80 | g | 0.8 | 5.879 | |
| Na-acetate | 2.00 | g | 2 | 24.38 | |
| Yeast extract | 1.00 | g | 1 | - | |
| Trace element solution (A) | 1.00 | ml | - | - | |
| MgCl2 x 7 H2O | 0.10 | g | 0.1 | 1.05 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| NH4Cl | 0.80 | g | 0.8 | 14.956 | |
| Na2CO3 | 21.20 | g | 21.2 | 200.023 | |
|
NaHCO3
(8 %) |
168.00 | ml | 13.44 | 159.987 | |
| Distilled water | 832.00 | ml | - | - | |
| Agar , for solid medium | 20.00 | g | 20 | 59.465 | |
| 1 We recommend that liquid and solid media be made by autoclaving the Na2CO3 separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na2CO3 otherwise the agar will be hydrolysed. When mixing the ingredients for media cool all components to about 55°C otherwise hydrolysis of the agar may occur. Adjust pH after autoclaving by adding the NaHCO3 (8 %) to pH 9.0. Please note that the NaHCO3 solution should be filter sterilized. | |||||
Solution occurs in 1
other media (first defined in Medium 253).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeCl2 x 4 H2O | 1.8 | g | 1.8 | 9.054 | |
| CoCl2 x 6 H2O | 250.0 | mg | 0.25 | 1.925 | |
| NiCl2 x 6 H2O | 10.0 | mg | 0.01 | 0.077 | |
| CuCl2 x 2 H2O | 10.0 | mg | 0.01 | 0.059 | |
| MnCl2 x 4 H2O | 70.0 | mg | 0.07 | 0.354 | |
| ZnCl2 | 100.0 | mg | 0.1 | 0.734 | |
| H3BO3 | 500.0 | mg | 0.5 | 8.085 | |
| Na2MoO4 x 2 H2O | 30.0 | mg | 0.03 | 0.124 | |
| Na2SeO3 x 5 H2O | 10.0 | mg | 0.01 | 0.038 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 For dissolving adjust pH to about 3 with 1 N HCl. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2CO3 | 21.2 | 200.023 |
| NaCl | 20 | 342.231 |
| Agar | 20 | 59.465 |
| NaHCO3 | 13.44 | 159.987 |
| Na-acetate | 2 | 24.38 |
| Show more | ||
