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Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract
(Difco, Bacto ) |
1.00 | g | 1 | - | |
Bacto Tryptone
(Difco) |
1.00 | g | 1 | - | |
Sodium glutamate monohydrate | 1.00 | g | 1 | 5.344 | |
CaSO4 x 2 H2O | 0.06 | g | 0.06 | 0.348 | |
MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
NaCl | 8.00 | mg | 0.008 | 0.137 | |
KNO3 | 0.10 | g | 0.1 | 0.989 | |
NaNO3 | 0.69 | g | 0.69 | 8.118 | |
Na2HPO4 | 0.11 | g | 0.11 | 0.775 | |
Trace element solution | 10.00 | ml | - | - | |
Agar | 20.00 | g | 20 | 59.465 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 7.5 with NaOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
12.80 | g | 12.8 | 66.967 | |
FeCl3 x 6 H2O | 1.35 | g | 1.35 | 4.995 | |
MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
CoCl2 x 6 H2O | 24.00 | mg | 0.024 | 0.185 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
CuCl2 x 2 H2O | 25.00 | mg | 0.025 | 0.147 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 24.00 | mg | 0.024 | 0.099 | |
NaCl | 1.00 | g | 1 | 17.112 | |
NiCl2 x 6 H2O | 0.12 | g | 0.12 | 0.926 | |
Na2SeO4 | 4.00 | mg | 0.004 | 0.021 | |
Na2WO4 | 4.00 | mg | 0.004 | 0.014 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve the nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0. |
Last modified: | 24.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.5 |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 20 | 59.465 |
Sodium glutamate monohydrate | 1 | 5.344 |
Yeast extract | 1 | - |
Bacto Tryptone | 1 | - |
NaNO3 | 0.69 | 8.118 |
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