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DSMZ

DSMZ 1474 . PROLIXIBACTER MEDIUM GYSW

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1474 #

ml
Equipment needed: Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.54 g 0.54 10.095
KH2PO4 0.14 g 0.14 1.029
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.15 g 0.15 1.02
Trace element solution 10.00 ml - -
Wolin's vitamin solution 10.00 ml - -
Resazurin solution 0.001 g/L 1.00 ml - -
D-Glucose 1.80 g 1.8 9.991
Yeast extract
(Difco) 		1.00 g 1 -
NaHCO3 2.52 g 2.52 29.998
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
Na2S x 9 H2O 0.50 g 0.5 2.082
Distilled water 1000.00 ml - -
1 Prepare the liquid medium without Vitamin solution, Cysteine-HCl x H2O and Na2S x 9 H2O. Adjust to pH 7.0 with 1 M NaOH. Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v). Sterilize by autoclaving. Before inoculation add from sterile stock solutions Cysteine-HCl x H2O, Vitamin solution (filter sterilized) and Na2S x 9 H2O.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnSO4 x H2O 0.100 g 0.1 0.592
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 5 H2O 0.025 g 0.025 0.111
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NaCl 1.000 g 1 17.112
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Na2SeO3 x 5 H2O 4.000 mg 0.004 0.015
Na2WO4 4.000 mg 0.004 0.014
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Resazurin solution 0.001 g/L #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Resazurin 1 mg 1.0e-3 0.004
Distilled water 1000 ml - -

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Filter, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.52 29.998
D-Glucose 1.8 9.991
Yeast extract 1 -
NH4Cl 0.54 10.095
L-Cysteine HCl x H2O 0.5 2.847
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains