1474 .
PROLIXIBACTER MEDIUM GYSW
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Equipment needed:
Filter
Hungate tubes
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 0.54 | g | ||
|
|
KH2PO4 | 0.14 | g | ||
|
|
MgCl2 x 6 H2O | 0.20 | g | ||
|
|
CaCl2 x 2 H2O | 0.15 | g | ||
|
|
Trace element solution | 10.00 | ml | ||
|
|
Wolin's vitamin solution | 10.00 | ml | ||
|
|
Resazurin solution 0.001 g/L | 1.00 | ml | ||
|
|
D-Glucose | 1.80 | g | ||
|
|
Yeast extract
(Difco) |
1.00 | g | ||
|
|
NaHCO3 | 2.52 | g | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
|
Na2S x 9 H2O | 0.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Prepare the liquid medium without Vitamin solution, Cysteine-HCl x H2O and Na2S x 9 H2O. Adjust to pH 7.0 with 1 M NaOH. Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v). Sterilize by autoclaving. Before inoculation add from sterile stock solutions Cysteine-HCl x H2O, Vitamin solution (filter sterilized) and Na2S x 9 H2O. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 12.800 | g | ||
|
|
FeCl3 x 6 H2O | 1.350 | g | ||
|
|
MnSO4 x H2O | 0.100 | g | ||
|
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CoCl2 x 6 H2O | 0.024 | g | ||
|
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CaCl2 x 2 H2O | 0.100 | g | ||
|
|
ZnCl2 | 0.100 | g | ||
|
|
CuCl2 x 5 H2O | 0.025 | g | ||
|
|
H3BO3 | 0.010 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.024 | g | ||
|
|
NaCl | 1.000 | g | ||
|
|
NiCl2 x 6 H2O | 0.120 | g | ||
|
|
Na2SeO3 x 5 H2O | 4.000 | mg | ||
|
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Na2WO4 | 4.000 | mg | ||
|
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Distilled water | 1000.000 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). | |||||
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium D-(+)-pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |