DSMZ 1475 . METALLIBACTERIUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 132.0 mg 0.132 0.999
MgCl2 x 6 H2O 53.0 mg 0.053 0.261
KH2PO4 27.0 mg 0.027 0.198
CaCl2 x 2 H2O 147.0 mg 0.147 1
VOSO4 x 5 H2O 0.5 mg 5.0e-4 0.002
Wolfe's mineral elixir 1.0 ml - -
FeSO4 x 7 H2O 10.0 mg 0.01 0.036
Trypticase peptone
(BD BBL)
1.4 g 1.4 -
Wolin's vitamin solution 10.0 ml - -
Distilled water 1000.0 ml - -
1 Dissolve ingredients except vitamins and ferrous sulfate and adjust pH of solution to 5.0. Dissolve ferrous sulfate (1% w/v) in 0.1 N H2SO4 and add to the medium prior to autoclaving. After autoclaving add vitamins from a stock solution that has been sterilized by filtration.
Solution occurs in 23 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 04.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 1.4 -
(NH4)2SO4 0.132 0.999
MgCl2 x 6 H2O 0.053 0.261
MgSO4 x 7 H2O 0.03 0.122
KH2PO4 0.027 0.198
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain