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DSMZ

DSMZ 1475 . METALLIBACTERIUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1475 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 132.0 mg 0.132 0.997
MgCl2 x 6 H2O 53.0 mg 0.053 0.26
KH2PO4 27.0 mg 0.027 0.198
CaCl2 x 2 H2O 147.0 mg 0.147 0.998
VOSO4 x 5 H2O 0.5 mg 5.0e-4 0.002
Wolfe's mineral elixir 1.0 ml - -
FeSO4 x 7 H2O 10.0 mg 0.01 0.036
Trypticase peptone
(BD BBL) 		1.4 g 1.397 -
Wolin's vitamin solution (10x) 1.0 ml - -
Distilled water 1000.0 ml - -
1 Dissolve ingredients except vitamins and ferrous sulfate and adjust pH of solution to 5.0. Dissolve ferrous sulfate (1% w/v) in 0.1 N H2SO4 and add to the medium prior to autoclaving. After autoclaving add vitamins from a stock solution that has been sterilized by filtration.

Wolfe's mineral elixir #

1000
Solution occurs in 21 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 04.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 1.397 -
(NH4)2SO4 0.132 0.997
MgCl2 x 6 H2O 0.053 0.26
MgSO4 x 7 H2O 0.03 0.121
KH2PO4 0.027 0.198
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain