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1475. METALLIBACTERIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 132.0 mg 0.131 0.988
2 53.0 mg 0.052 0.258
3 27.0 mg 0.027 0.196
4 147.0 mg 0.145 0.989
5 0.5 mg 4.9e-4 0.002
6 Wolfe's mineral elixir 1.0 ml - -
7 10.0 mg 0.01 0.036
8
(BD BBL)
1.4 g 1.385 -
9 Vitamin solution 10.0 ml - -
10 1000.0 ml 987.346 54806
11 , required for dissolving ferrous chloride 0.1 N - -
Instructions:
  1. Dissolve ingredients except vitamins and ferrous sulfate and adjust pH of solution to 5.0. Dissolve ferrous sulfate (1% w/v) in 0.1 N H2SO4 and add to the medium prior to autoclaving. After autoclaving add vitamins from a stock solution that has been sterilized by filtration.
Solution occurs in 21 other media (first defined in medium 792 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 30 121.716
2 5.00 g 5 29.583
3 10.00 g 10 171.108
4 1.00 g 1 3.597
5 1.80 g 1.8 7.565
6 1.00 g 1 6.802
7 1.80 g 1.8 6.26
8 0.10 g 0.1 0.401
9 0.18 g 0.18 0.379
10 0.10 g 0.1 1.617
11 0.10 g 0.1 0.413
12 2.80 g 2.8 7.089
13 0.10 g 0.1 0.303
14 0.10 g 0.1 0.529
15 1000.00 ml 998.207 55408.8
Instructions:
  1. First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.0
Equipment needed: Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
Trypticase peptone 1.385 -
CaCl2 x 2 H2O 0.146 0.996
(NH4)2SO4 0.131 0.988
MgCl2 x 6 H2O 0.052 0.258
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Cultivation metadata from 1 strains BacDive

halophily not 1 strain
pH 0 - 2 1 strain
pH 2 - 4 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
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