1475 .
METALLIBACTERIUM MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
5.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 132.0 | mg | ||
|
|
MgCl2 x 6 H2O | 53.0 | mg | ||
|
|
KH2PO4 | 27.0 | mg | ||
|
|
CaCl2 x 2 H2O | 147.0 | mg | ||
|
|
VOSO4 x 5 H2O | 0.5 | mg | ||
|
|
Wolfe's mineral elixir | 1.0 | ml | ||
|
|
FeSO4 x 7 H2O | 10.0 | mg | ||
|
|
Trypticase peptone
(BD BBL) |
1.4 | g | ||
|
|
Wolin's vitamin solution (10x) | 1.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve ingredients except vitamins and ferrous sulfate and adjust pH of solution to 5.0. Dissolve ferrous sulfate (1% w/v) in 0.1 N H2SO4 and add to the medium prior to autoclaving. After autoclaving add vitamins from a stock solution that has been sterilized by filtration. | |||||
Wolfe's mineral elixir #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 30.00 | g | ||
|
|
MnSO4 x H2O | 5.00 | g | ||
|
|
NaCl | 10.00 | g | ||
|
|
FeSO4 x 7 H2O | 1.00 | g | ||
|
|
CoCl2 x 6 H2O | 1.80 | g | ||
|
|
CaCl2 x 2 H2O | 1.00 | g | ||
|
|
ZnSO4 x 7 H2O | 1.80 | g | ||
|
|
CuSO4 x 5 H2O | 0.10 | g | ||
|
|
AlK(SO4)2 x 12 H2O | 0.18 | g | ||
|
|
H3BO3 | 0.10 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.10 | g | ||
|
|
(NH4)2Ni(SO4)2 x 6 H2O | 2.80 | g | ||
|
|
Na2WO4 x 2 H2O | 0.10 | g | ||
|
|
Na2SeO4 | 0.10 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts. | |||||
Wolin's vitamin solution (10x) #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 20 | mg |
|
|
Folic acid | 20 | mg |
|
|
Pyridoxine hydrochloride | 100 | mg |
|
|
Thiamine HCl | 50 | mg |
|
|
Riboflavin | 50 | mg |
|
|
Nicotinic acid | 50 | mg |
|
|
Calcium D-(+)-pantothenate | 50 | mg |
|
|
Vitamin B12 | 1 | mg |
|
|
p-Aminobenzoic acid | 50 | mg |
|
|
(DL)-alpha-Lipoic acid | 50 | mg |
|
|
Distilled water | 1000 | ml |