1477
1477 .
CALORAMATOR QUIMBAYENSIS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1477 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.3 | g | 0.299 | 1.719 | |
| KH2PO4 | 0.3 | g | 0.299 | 2.2 | |
| NH4Cl | 1.0 | g | 0.998 | 18.658 | |
| MgCl2 x 6 H2O | 1.5 | g | 1.497 | 7.363 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.679 | |
| NaCl | 20.0 | g | 19.96 | 341.548 | |
| KCl | 0.1 | g | 0.1 | 1.339 | |
| Trace element solution | 1.0 | ml | - | - | |
| Yeast extract | 1.0 | g | 0.998 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 0.5 | g | 0.499 | 5.94 | |
| D-Glucose | 3.6 | g | 3.593 | 19.942 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except bicarbonate, glucose and vitamins, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose and vitamins (sterilized by filtration) from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary. | |||||
Solution occurs in 14
other media (first defined in Medium 318).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
12.80 | g | 12.8 | 66.967 | |
| FeCl2 x 4 H2O | 1.00 | g | 1 | 5.03 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
| CoCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
| CuCl2 | 0.02 | g | 0.02 | 0.149 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| NiCl2 x 6 H2O | 0.10 | g | 0.1 | 0.772 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| Na2SeO3 x 5 H2O | 0.03 | g | 0.03 | 0.114 | |
| Na2WO4 x 2 H2O | 0.04 | g | 0.04 | 0.121 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml. | |||||
Solution occurs in 305
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 04.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| D-Glucose | 3.593 | 19.942 |
| MgCl2 x 6 H2O | 1.497 | 7.363 |
| NH4Cl | 0.998 | 18.658 |
| Yeast extract | 0.998 | - |
| NaHCO3 | 0.499 | 5.94 |
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