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DSMZ

DSMZ 1478 . NATRANAEROARCHAEUM MEDIUM AARC1

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1478 #

ml
Equipment needed: Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 72.000 g 72 1232.03
K2HPO4 2.500 g 2.5 14.353
(NH4)2SO4 0.125 g 0.125 0.946
Na2CO3 142.500 g 142.5 1344.49
NaHCO3 22.500 g 22.5 267.835
Trace element solution 1.000 ml - -
Sulfur powder 2.000 g 2 7.797
Distilled water 1000.000 ml - -
1 Prepare the liquid medium. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Steam medium for 3h on each of 3 successive days. Before inoculation add from sterile stock solutions:
MgCl2 x 6 H2O 1.200 mM 0.244 1.2
NaHCO3 1.250 mM 0.105 1.25
NH4Cl 3.000 mM 0.16 3
Sodium formate 50.000 mM 3.4 50
Sodium acetate 2.000 mM 0.164 2
Yeast extract 20.000 mg/l 0.02 -
Vitamine mixture 1.000 ml - -
Disodium sulfide
(1M filter sterilized stock) 		1.000 mM - 1

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 1478).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 5 H2O 0.01 g 0.01 0.045
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 Adjust pH to 3.0-4.0 with HCl.
2 Autoclave at 121 °C for 15 min.
3 Note: autoclave in closed bottles

Vitamine mixture #

400
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acidic thiamine / Ca pantothenate solution 100 ml - -
Basic vitamin solution 100 ml - -
Neutral folic acid / riboflavin solution 100 ml - -
Neutral B12 solution 0.005 g/L 100 ml - -
1 Sterilize by filtration.
2 Mix the four solutions in the ratio 1:1:1:1.

Acidic thiamine / Ca pantothenate solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine 5 mg 0.05 0.188
Calcium pantothenate 5 mg 0.05 0.105
Distilled water
(acidified to pH 3.0 with HCl) 		100 ml - -

Basic vitamin solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2 mg 0.02 0.082
PABA
(p-aminobenzoic acid) 		5 mg 0.05 0.365
Nicotinic acid 5 mg 0.05 0.406
Pyridoxine 10 mg 0.1 0.591
NaOH
(0.1N) 		100 ml - -

Neutral folic acid / riboflavin solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Folic acid 2 mg 0.02 0.045
Riboflavin 5 mg 0.05 0.133
Distilled water 100 ml - -

Neutral B12 solution 0.005 g/L #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
B12 0.5 mg 0.005 0.004
Distilled water 100.0 ml - -

Metadata

Last modified: 24.04.23
Source: DSMZ
Medium type: Complex medium
Final pH: 3.0 - 4.0
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 142.5 1344.49
NaCl 72 1232.03
Sodium formate 3.4 50
K2HPO4 2.5 14.353
Sulfur powder 2 7.797
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