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148. DESULFUROMONAS SP. MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.0 g 0.996 7.319
2 0.5 g 0.498 9.31
3 0.4 g 0.398 1.616
4 0.1 g 0.1 0.677
5 Trace element solution SL-10 1.0 ml - -
6 Selenite-tungstate solution 1.0 ml - -
7 0.5 g 0.498 6.071
8 1.5 g 1.494 12.872
9
(0.1% w/v)
0.5 ml 5.0e-4 0.002
10 1.5 g 1.494 14.096
11 Seven vitamins solution 1.0 ml - -
12 0.5 g 0.498 2.073
13 1000.0 ml 994.23 55188.1
Instructions:
  1. Dissolve ingredients (except carbonate, vitamins and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials. After autoclaving complete the medium by adding vitamins and sulfide from separately sterilized anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins are sterilized by filtration. Adjust pH of the complete medium to 7.0-7.2, if necessary.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1
Equipment needed: Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.203 55353.1
Na2CO3 1.494 14.096
Fumaric acid 1.494 12.872
KH2PO4 0.996 7.319
NH4Cl 0.498 9.31
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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503. ANAEROBIC FRESHWATER (FWM) MEDIUM 86 %
1318. METHANOCELLA CONRADII MEDIUM 85 %

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