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DSMZ

DSMZ 1483 . HALARCHAEUM MEDIUM RS94

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1483 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 200.0 g 200 3422.31
MgCl2 x 6 H2O 25.0 g 25 122.969
K2SO4 5.0 g 5 28.693
CaCl2 x 2 H2O 0.1 g 0.1 0.68
NaH2PO4 x 2 H2O 0.2 g 0.2 1.667
Na2HPO4 x 12 H2O 0.4 g 0.4 2.818
Na pyruvate 2.0 g 2 18.175
Na acetate 0.5 g 0.5 6.095
Na malate 0.5 g 0.5 2.808
Casein hydrolysate
(Oxoid LP0041) 		2.0 g 2 -
Yeast extract
(Fisher BP1422-500) 		1.0 g 1 -
Tryptone
(BD211921) 		0.5 g 0.5 7.034
Glucose 0.5 g 0.5 2.775
Trace elements solution (Pfennig & Lippert,1966) 1.0 ml - -
Agar 20.0 g 20 59.465
Distilled water 1000.0 ml - -
1 Adjust to pH 7.1

Trace elements solution (Pfennig & Lippert,1966) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.1
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 200 3422.31
MgCl2 x 6 H2O 25 122.969
Agar 20 59.465
K2SO4 5 28.693
Na pyruvate 2 18.175
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain