DSMZ 1484 . Glycerol-Yeast extract-Peptone Medium

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto peptone 3.00 g 3 -
Yeast extract 5.00 g 5 -
Betaine 1.25 g 1.25 10.67
Sodium pyruvate 1.25 g 1.25 11.36
Agar , for solid medium 15.00 g 15 44.599
Modified Wolin's mineral solution 10.00 ml - -
Wolin's vitamin solution 10.00 ml - -
Glycerol 10.00 ml - -
Distilled water 970.00 ml - -
1 Adjust to pH 7.2
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 170 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 27.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 37

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Yeast extract 5 -
Bacto peptone 3 -
Sodium pyruvate 1.25 11.36
Betaine 1.25 10.67
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Cultivation metadata from 37 strains BacDive

pH 6 - 8 11 strains
pH 8 - 10 7 strains
pH 4 - 6 5 strains
phyla 4 35 strains
oxygen aerobe 11 strains
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