Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 16.50 | g | 16.321 | 279.269 | |
MgCl2 x 6 H2O | 3.50 | g | 3.462 | 17.028 | |
Na2SO4 | 2.75 | g | 2.72 | 19.15 | |
KCl | 0.50 | g | 0.495 | 6.634 | |
NaNO3 | 0.50 | g | 0.495 | 5.819 | |
CaCl2 x 2 H2O | 0.40 | g | 0.396 | 2.691 | |
NH4Cl | 0.25 | g | 0.247 | 4.623 | |
Trace element solution | 10.00 | ml | - | - | |
Na-acetate | 0.25 | g | 0.247 | 3.014 | |
Disodium succinate | 0.50 | g | 0.495 | 3.052 | |
Casamino acids
(BD Bacto) |
0.50 | g | 0.495 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
K2HPO4 | 0.15 | g | 0.148 | 0.852 | |
Na2CO3 | 1.50 | g | 1.484 | 13.999 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.25 | g | 0.247 | 1.408 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except hydrogenphosphate, carbonate, vitamins and cysteine), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add hydrogenphosphate, vitamins and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
12.80 | g | 12.8 | 66.967 | |
FeCl2 x 4 H2O | 1.00 | g | 1 | 5.03 | |
MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
CoCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
CuCl2 | 0.02 | g | 0.02 | 0.149 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
NiCl2 x 6 H2O | 0.10 | g | 0.1 | 0.772 | |
NaCl | 1.00 | g | 1 | 17.112 | |
Na2SeO3 x 5 H2O | 0.03 | g | 0.03 | 0.114 | |
Na2WO4 x 2 H2O | 0.04 | g | 0.04 | 0.121 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 04.05.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 - 7.2 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MgCl2 x 6 H2O | 3.462 | 17.028 |
Na2SO4 | 2.72 | 19.15 |
Na2CO3 | 1.484 | 13.999 |
KCl | 0.495 | 6.634 |
NaNO3 | 0.495 | 5.819 |
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