1492
1492 .
HALF STRENGTH R2A
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for DSM 25439
* Modification: Use 2% (w/v) gellan gum as solidifying agent.
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 60 °C
Main sol. 1492 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.250 | g | 0.25 | - | |
|
Proteose peptone
(Difco no. 3) |
0.250 | g | 0.25 | - | |
| Casamino acids | 0.250 | g | 0.25 | - | |
| Glucose | 0.250 | g | 0.25 | 1.388 | |
|
Starch
(soluble) |
0.250 | g | 0.25 | - | |
| Na-pyruvate | 0.250 | g | 0.25 | 2.272 | |
| K2HPO4 | 0.150 | g | 0.15 | 0.861 | |
| MgSO4 x 7 H2O | 0.025 | g | 0.025 | 0.101 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Adjust pH to 7.2 with K2HPO4 or KH2PO4. | |||||
| 2 Add agar after adjusting the pH, then boil to dissolve the agar | |||||
| Agar* | 15.000 | g | 15 | - | |
| Gellan Gum* | 2.000 | %(w/v) | - | - | |
| 3 Autoclave at 121 °C for 15 min. | |||||
Metadata
| Last modified: | 03.01.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Gellan Gum | 20 | - |
| Na-pyruvate | 0.25 | 2.272 |
| Glucose | 0.25 | 1.388 |
| Casamino acids | 0.25 | - |
| Starch | 0.25 | - |
| Show more | ||
