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DSMZ

DSMZ 1493 . VERRUCOMICROBIA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1493 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 0.50 g 0.5 5.952
NaCl 0.50 g 0.5 8.556
KH2PO4 0.40 g 0.4 2.939
(NH4)2SO4 0.20 g 0.2 1.514
MgSO4 x 6 H2O 0.10 g 0.1 0.831
CaCl2 0.01 g 0.01 0.09
Li2B4O7 0.01 g 0.01 -
FeEDTA solution 1.00 ml - -
Trace element solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Do not adjust the pH.
2 For plates add 8g/l Gelrite or 15g Phytagel and 1g/L MgCl. A carbon source is not required because the organism degrades the polysaccharides in Gelrite or Phytagel
3 For liquid medium use 0.8-2.0g/l Gelrite as carbon source (the medium remains liquid). Alternatively mannose (1g/l) may be used instead of Gelrite in liquid medium. Do not add mannose to solid media, which will inhibit growth.

FeEDTA solution #

1000
Solution occurs in 1 other media (first defined in Medium 1493).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 1.54 g 1.54 5.539
Na-EDTA 2.06 g 2.06 6.127
Distilled water 1000.00 ml - -

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 1493).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
Fe(NH4)2(SO4)2 x 6 H2O 0.200 g 0.2 -
Na2SeO4 x 10 H2O 0.440 g 0.44 -
CoCl2 x 6 H2O 0.180 g 0.18 1.386
MnSO4 x H2O 0.120 g 0.12 0.71
Na2MoO4 0.160 g 0.16 0.777
Na2WO4 0.100 g 0.1 0.34
ZnSO4 x 7 H2O 0.540 g 0.54 1.878
AlCl3 0.040 g 0.04 0.3
NiCl2 x 6 H2O 0.025 g 0.025 0.193
H3BO3 0.110 g 0.11 1.779
CuSO4 0.210 g 0.21 1.316
MnCl2 0.190 g 0.19 1.51
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
2 Final pH 7.0 (with KOH).

Metadata

Last modified: 08.09.22
Source: DSMZ
Synonyms: . VERRUCOMICROBIA MEDIUM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.5 8.556
NaHCO3 0.5 5.952
KH2PO4 0.4 2.939
(NH4)2SO4 0.2 1.514
MgSO4 x 6 H2O 0.1 0.831
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain