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14. CLOSTRIDIUM FORMICOACETICUM MEDIUM

Culture medium recipe

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* Modified for Clostridium formicaceticum DSM 92
beta

Anaerobic

Equipment needed: Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 4.941 -
2 10.00 g 9.881 56.732
3 Trace element solution SL-4 10.00 ml - -
4
(0.1% w/v)
1.00 ml 9.9e-4 0.005
5 0.75 g 0.741 6.495
6
(0.1% w/v)
0.50 ml 4.9e-4 0.002
7 1.00 g 0.988 9.323
8 5.00 g 4.941 27.425
9
optional
15.00 g 14.822 -
10 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients (except carbonate and fructose) and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 8.0.
Solution occurs in 13 other media (first defined in medium 14 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.5 1.487
2 0.20 g 0.2 0.719
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.30 g 0.3 4.852
6 0.20 g 0.2 0.841
7 0.01 g 0.01 0.059
8 0.02 g 0.02 0.084
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.0
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 8
Strains with modifications: 8
Clostridium formicaceticum DSM 92

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.235 55299.3
Agar
optional
14.822 -
K2HPO4 9.881 56.732
D-Fructose 4.941 27.425
Yeast extract 4.941 -
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Cultivation metadata from 8 strains BacDive

phyla 1 8 strains
oxygen anaerobe 8 strains
temperature mesophilic 8 strains

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