This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution 1 (10x NMS salts) | 100.0 | ml | - | - | |
NaCl
(2 %) |
900.0 | ml | 18 | 308.008 | |
Solution 2 (Fe EDTA) | 0.1 | ml | - | - | |
Solution 3 (Sodium molybdate) | 1.0 | ml | - | - | |
Trace elements | 1.0 | ml | - | - | |
Agar , for solid medium | 1.5 | % | 15 | 44.599 | |
Phosphate buffer | 10.0 | ml | - | - | |
1 Prepare the growth medium as follows: | |||||
2 Dilute 100 ml solution 1 to 1 litre with 2% NaCl and then add 1 ml of solution 3, 1 ml of the trace elements, and 0.1 ml of solution 2. If solid media is required add 1.5% agar. Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 25% methane to the gas phase and autoclave at 121C (15psi) for 15 minutes. Autoclave the phosphate buffer separately. When the growth medium is cool 10 ml/l of the phosphate buffer is added - if too warm the phosphate will precipitate. Liquid cultures should be grown with shaking. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 25.0 | mg | 0.25 | 0.744 | |
CuSO4 x 5 H2O | 20.0 | mg | 0.2 | 0.801 | |
FeSO4 x 7 H2O | 50.0 | mg | 0.5 | 1.798 | |
ZnSO4 x 7 H2O | 40.0 | mg | 0.4 | 1.391 | |
H3BO3 | 1.5 | mg | 0.015 | 0.243 | |
CoCl2 x 6 H2O | 5.0 | mg | 0.05 | 0.385 | |
MnCl2 x 4 H2O | 2.0 | mg | 0.02 | 0.101 | |
NiCl2 x 6 H2O | 1.0 | mg | 0.01 | 0.077 | |
Distilled water | 100.0 | ml | - | - | |
1 May be stored at 4°C in the dark |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2HPO4 x 12 H2O | 71.6 | g | 71.6 | 504.371 | |
KH2PO4 | 26.0 | g | 26 | 191.057 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve in about 800 ml of water, adjust the pH to 6.8 and make up to 1 litre. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KNO3 | 10 | g | 10 | 98.909 | |
MgSO4 x 6 H2O | 10 | g | 10 | 83.078 | |
CaCl2 x 2 H2O | 2 | g | 2 | 13.604 | |
Distilled water | 1000 | ml | - | - | |
1 Dissolve the ingredients listed above (in that order) in about 700 ml of 2% NaCl, and then make up to 1 litre with 2% NaCl. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Fe(III)-EDTA | 3.8 | g | 38 | 110.447 |
Distilled water | 100.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2MoO4 x 2 H2O | 0.26 | g | 0.26 | 1.075 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 15.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | n.d. |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 18 | 308.008 |
Agar | 15 | 44.599 |
KNO3 | 1 | 9.891 |
MgSO4 x 6 H2O | 1 | 8.308 |
Na2HPO4 x 12 H2O | 0.716 | 5.044 |
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phyla | 1 | 1 strain |