1505
1505 .
PROTEINIVORAX MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1505 #
ml
Equipment needed: Autoclave
Filter
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.3 | 2.205 | |
| MgSO4 x 7 H2O | 0.12 | g | 0.12 | 0.487 | |
| CaCl2 x 2 H2O | 0.03 | g | 0.03 | 0.204 | |
| NaCl | 20.00 | g | 20 | 342.231 | |
|
Tryptone
(BD Bacto) |
3.00 | g | 3 | 42.206 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Na2CO3 | 60.00 | g | 60 | 566.102 | |
| NaHCO3 | 50.00 | g | 50 | 595.188 | |
| Na-thioglycolate | 1.00 | g | 1 | 8.764 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonates and thioglycolate), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate and hydrogencarbonate, adjust pH to 8.5 - 9.0, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add thioglycolate from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 04.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 8.5 - 9.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2CO3 | 60 | 566.102 |
| NaHCO3 | 50 | 595.188 |
| NaCl | 20 | 342.231 |
| Tryptone | 3 | 42.206 |
| Na-thioglycolate | 1 | 8.764 |
| Show more | ||
