150a
150a .
ACIDITHIOBACILLUS CALDUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acidithiobacillus caldus DSM 8584
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
45 °C
Main sol. 150a #
ml
Equipment needed: Autoclave
Filter
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 3.00 | g | 2.97 | 22.478 | |
| K2HPO4 x 3 H2O | 0.50 | g | 0.495 | 2.842 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.495 | 2.009 | |
| KCl | 0.10 | g | 0.099 | 1.328 | |
| Ca(NO3)2 x 4 H2O | 0.02 | g | 0.02 | 0.084 | |
| Trace element solution | 10.00 | ml | - | - | |
|
Sulfur
(powdered) |
5.00 | g | 4.951 | 154.365 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except trace elements and sulfur. Adjust pH with 6 N H2SO4 to 2.5, dispense into suitable cultivation vessels and autoclave. After autoclaving add filter-sterilized trace elements solution (see below) and sterile sulfur powder. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeCl3 x 6 H2O | 1.10 | g | 1.1 | 4.07 |
| CuSO4 x 5 H2O | 0.05 | g | 0.05 | 0.2 |
| H3BO3 | 0.20 | g | 0.2 | 3.234 |
| MnSO4 x H2O | 0.20 | g | 0.2 | 1.183 |
| Na2MoO4 x 2 H2O | 0.08 | g | 0.08 | 0.331 |
| CoCl2 x 6 H2O | 0.06 | g | 0.06 | 0.462 |
| ZnSO4 x 7 H2O | 0.09 | g | 0.09 | 0.313 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 24.07.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 2.5 |
| Equipment needed: | Autoclave, Filter, Water bath |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Acidithiobacillus caldus DSM 8584
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 4.951 | 154.365 |
| (NH4)2SO4 | 2.97 | 22.478 |
| K2HPO4 x 3 H2O | 0.495 | 2.842 |
| MgSO4 x 7 H2O | 0.495 | 2.009 |
| KCl | 0.099 | 1.328 |
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