1511 .
PLANTOMYCETACEAE MEDIUM SBP2
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Equipment needed:
Hungate tubes
Final pH:
7.6 - 8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.33 | g | ||
|
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NH4Cl | 0.33 | g | ||
|
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MgCl2 | 1.20 | mg | ||
|
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CaCl2 | 1.20 | mg | ||
|
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KCl | 0.33 | g | ||
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Trace Element Solution | 1.00 | ml | ||
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Wolin's vitamin solution | 1.00 | ml | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Prepare the liquid medium. Dispense the medium into culture vessels. Sterilize by autoclaving. Before inoculation add from sterile stock solutions: | |||||
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KNO3 | 1.00 | g | ||
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Glucose | 2.00 | g | ||
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Wolin's vitamin solution | 1.00 | ml | ||
|
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NaHCO3
(8% solution) |
12.50 | ml | ||
| 2 The 8% NaHCO3 solution may be either filter sterilized or autoclaved. When the solution is autoclaved use tightly closed vessels that are filled to a maximum of 50% of the total volume. | |||||
| 3 The final pH of the medium should be adjusted to pH 7.6-8.0. | |||||
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
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Pyridoxine hydrochloride | 10.0 | mg |
|
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
|
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Nicotinic acid | 5.0 | mg |
|
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Calcium D-(+)-pantothenate | 5.0 | mg |
|
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Vitamin B12 | 0.1 | mg |
|
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p-Aminobenzoic acid | 5.0 | mg |
|
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace Element Solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Fe(NH4)2(SO4)2 x 6 H2O | 784 | mg |
|
|
NiCl2 x 2 H2O | 284 | mg |
|
|
CoCl2 x 6 H2O | 38 | mg |
|
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MnCl2 x 4 H2O | 99 | mg |
|
|
ZnSO4 x 7 H2O | 144 | mg |
|
|
Na2SeO4 | 95 | mg |
|
|
Na2WO4 x 2 H2O | 33 | mg |
|
|
Na2MoO4 x 2 H2O | 24 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
Distilled water | 1000 | ml |