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1513. SULFURIMONAS GOTLANDICA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.55 g 5.383 92.11
2 2.29 g 2.221 10.925
3 0.34 g 0.33 2.243
4 0.15 g 0.145 1.952
5 0.91 g 0.883 6.214
6 1.01 g 0.98 9.689
7 2.38 g 2.308 -
8 0.05 g 0.048 0.907
9
(0.1% w/v)
8.00 ml 0.008 0.049
10
(0.1% w/v)
6.00 ml 0.006 0.094
11
(0.1% w/v)
4.00 ml 0.004 0.092
12
(0.1% w/v)
1.00 ml 9.7e-4 0.006
13 Trace element solution SL-10 1.00 ml - -
14 Selenite-tungstate solution 1.00 ml - -
15 0.20 g 0.194 2.309
16 Vitamin solution 10.00 ml - -
17 2.48 g 2.405 9.692
18 1000.00 ml 968.193 53742.8
Instructions:
  1. Dissolve ingredients (except bicarbonate, vitamins and thiosulfate) and adjust pH to 7.3. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., Balch-type tubes) and autoclave. Add vitamins and thiosulfate from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and thiosulftae should be sterilized by filtration. Adjust pH of the complete medium to 7.3 - 7.5, if necessary. After inoculation pressurize vials to 0.7 bar overpressure with sterile 100% N2 gas.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 979.802 54387.2
NaCl 5.383 92.11
Na2S2O3 x 5 H2O 2.405 9.692
HEPES 2.308 -
MgCl2 x 6 H2O 2.221 10.925
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Cultivation metadata from 1 strains BacDive

halophily not 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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