1515
1515 .
OCEANIRHABDUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1515 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 1.0 | g | 1 | 7.04 | |
| NaCl | 30.0 | g | 30 | 513.347 | |
| KCl | 0.7 | g | 0.7 | 9.39 | |
| MgCl2 x 6 H2O | 5.0 | g | 5 | 24.594 | |
| CaCl2 x 2 H2O | 1.0 | g | 1 | 6.802 | |
| K2HPO4 | 0.4 | g | 0.4 | 2.297 | |
| KH2PO4 | 0.4 | g | 0.4 | 2.939 | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
|
Fe(NH4)2(SO4)2 x 6 H2O
(0.1% w/v) |
2.0 | ml | 0.002 | - | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
|
Yeast extract
(OXOID) |
3.0 | g | 3 | - | |
|
Tryptone
(BD Bacto) |
4.0 | g | 4 | 56.275 | |
| Na-acetate | 6.8 | g | 6.8 | 82.893 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.6 | g | 0.6 | 3.416 | |
| NaHCO3 | 2.5 | g | 2.5 | 29.759 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except cysteine and bicarbonate, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve cysteine, adjust pH to 6.5, then dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.5 - 7.0, if necessary. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 04.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.5 - 7.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 30 | 513.347 |
| Na-acetate | 6.8 | 82.893 |
| MgCl2 x 6 H2O | 5 | 24.594 |
| Tryptone | 4 | 56.275 |
| Yeast extract | 3 | - |
| Show more | ||
