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DSMZ

DSMZ 1516 . THERMOFILUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1516 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Liquid medium 1000.0 ml - -
1 Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
Filtrate of Desulfurococcus kamchatkensis (DSM18924) 10.0 ml - -
Wolin's vitamin solution 10.0 ml - -
Na2S x H2O 0.3 g 0.3 3.844
2 Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:

Trace Element Solution #

1000
Equipment needed: Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(NH4)2(SO4)2 x 6 H2O 784 mg 0.784 -
NiCl2 x 2 H2O 225 mg 0.225 1.736
CoCl2 x 6 H2O 38 mg 0.038 0.293
MnCl2 x 4 H2O 99 mg 0.099 0.5
ZnSO4 x 7 H2O 144 mg 0.144 0.501
Na2SeO4 95 mg 0.095 0.503
Na2WO4 x 2 H2O 33 mg 0.033 0.1
Na2MoO4 x 2 H2O 24 mg 0.024 0.099
H3BO3 6 mg 0.006 0.097
CuCl2 x 2 H2O 2 mg 0.002 0.012
Distilled water 1000 ml - -

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Filtrate of Desulfurococcus kamchatkensis (DSM18924) #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Liquid medium 10.0 ml - -
Glucose 2.0 g/l 2 11.101
Yeast extract 0.6 g/l 0.6 -
1 Strain DSM 18924 was inoculated into anaerobic tube, containing 10ml of the medium mentioned above. Glucose (2g/l) and yeast extract (0,6g/l) were added as substrate. After 3 days of incubation culture was filtered throuth 0,22nm filter. Filtered culture broth is used for the cultivation of the strain at a concentration of 1% as a source of growth factor

Liquid medium #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.33 g 0.33 2.425
NH4Cl 0.33 g 0.33 6.169
MgCl2 0.33 g 0.33 3.466
CaCl2 0.33 g 0.33 2.974
KCl 0.33 g 0.33 4.427
Glucose 2.00 g 2 11.101
Yeast extract 0.10 g 0.1 -
Trace Element Solution 1.00 ml - -
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.8 - 6.2
Equipment needed: Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 2 11.101
NH4Cl 0.33 6.169
KCl 0.33 4.427
MgCl2 0.33 3.466
CaCl2 0.33 2.974
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature hyperthermophilic 1 strain