This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Mineral salt solution | 20.00 | ml | - | - | |
Peptone
(Bacto) |
0.50 | g | 0.336 | - | |
Glucose | 0.25 | g | 0.168 | 0.931 | |
HEPES
(10mM) |
2.38 | g | 1.597 | 6.703 | |
Artifical sea water | 500.00 | ml | - | - | |
Agar
(Bacto) |
15.00 | g | 10.067 | 29.932 | |
Distilled water | 475.00 | ml | - | - | |
1 Sterilize 20 min/121°C. After cooling to 60°C add to the medium: | |||||
Vitamin solution (double conc.) | 5.00 | ml | - | - | |
2 Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
10.00 | g | 10.526 | 55.071 | |
MgSO4 x 7 H2O | 29.70 | g | 31.263 | 126.841 | |
CaCl2 x 2 H2O | 3.34 | g | 3.516 | 23.915 | |
Na2MoO4 x 2 H2O | 12.67 | mg | 0.013 | 0.055 | |
FeSO4 x 7 H2O | 99.00 | mg | 0.104 | 0.375 | |
Metall salt sol. 44
(see medium 590) |
50.00 | ml | - | - | |
Distilled water | 900.00 | ml | - | - | |
1 Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Biotin | 4.0 | mg | 0.004 | 0.016 | |
Folic acid | 4.0 | mg | 0.004 | 0.009 | |
Pyridoxine hydrochloride | 20.0 | mg | 0.02 | 0.097 | |
Riboflavine | 10.0 | mg | 0.01 | 0.027 | |
Thiamine-HCl x 2 H2O | 10.0 | mg | 0.01 | 0.027 | |
Nicotinamide | 10.0 | mg | 0.01 | 0.082 | |
D-Calcium pantothenate | 10.0 | mg | 0.01 | - | |
Vitamin B12 | 0.2 | mg | 2.0e-4 | 1.5e-4 | |
p-Aminobenzoic acid | 10.0 | mg | 0.01 | 0.073 | |
Distilled water | 1000.0 | ml | - | - | |
1 Store in the dark and cold (5°C). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 23.47 | g | 23.47 | 401.608 |
Na2SO4 | 3.92 | g | 3.92 | 27.598 |
MgCl2 x 6 H2O | 4.98 | g | 4.98 | 24.496 |
CaCl2 | 1.10 | g | 1.1 | 9.912 |
NaHCO3 | 192.00 | mg | 0.192 | 2.286 |
KCl | 664.00 | mg | 0.664 | 8.907 |
KBr | 96.00 | mg | 0.096 | 0.807 |
H3BO3 | 26.00 | mg | 0.026 | 0.42 |
SrCl2 | 24.00 | mg | 0.024 | 0.151 |
NaF | 3.00 | mg | 0.003 | 0.071 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 11.10.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.5 |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 10.067 | 29.932 |
NaCl | 7.876 | 134.768 |
MgCl2 x 6 H2O | 3.57 | 17.562 |
HEPES | 1.597 | 6.703 |
Na2SO4 | 1.315 | 9.261 |
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