DSMZ 1524 . PLANCTOMYCETES MEDIUM NH11

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral salt solution 20.00 ml - -
Peptone
(Bacto)
0.50 g 0.336 -
Glucose 0.25 g 0.168 0.931
HEPES
(10mM)
2.38 g 1.597 6.703
Artifical sea water 500.00 ml - -
Agar
(Bacto)
15.00 g 10.067 29.932
Distilled water 475.00 ml - -
1 Sterilize 20 min/121°C. After cooling to 60°C add to the medium:
Vitamin solution (double conc.) 5.00 ml - -
2 Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).
Solution occurs in 3 other media (first defined in Medium 621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.00 g 10.526 55.071
MgSO4 x 7 H2O 29.70 g 31.263 126.841
CaCl2 x 2 H2O 3.34 g 3.516 23.915
Na2MoO4 x 2 H2O 12.67 mg 0.013 0.055
FeSO4 x 7 H2O 99.00 mg 0.104 0.375
Metall salt sol. 44
(see medium 590)
50.00 ml - -
Distilled water 900.00 ml - -
1 Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water.
Solution occurs in 6 other media (first defined in Medium 621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.0 mg 0.004 0.016
Folic acid 4.0 mg 0.004 0.009
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Riboflavine 10.0 mg 0.01 0.027
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
D-Calcium pantothenate 10.0 mg 0.01 -
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Distilled water 1000.0 ml - -
1 Store in the dark and cold (5°C).
Solution occurs in 1 other media (first defined in Medium 600a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 23.47 g 23.47 401.608
Na2SO4 3.92 g 3.92 27.598
MgCl2 x 6 H2O 4.98 g 4.98 24.496
CaCl2 1.10 g 1.1 9.912
NaHCO3 192.00 mg 0.192 2.286
KCl 664.00 mg 0.664 8.907
KBr 96.00 mg 0.096 0.807
H3BO3 26.00 mg 0.026 0.42
SrCl2 24.00 mg 0.024 0.151
NaF 3.00 mg 0.003 0.071
Distilled water 1000.00 ml - -

Metadata

Last modified: 11.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 10.067 29.932
NaCl 7.876 134.768
MgCl2 x 6 H2O 3.57 17.562
HEPES 1.597 6.703
Na2SO4 1.315 9.261
Show more

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature psychrophilic 1 strain