Loading ...

1526c. DESULFOHALOVIBRIO (L21 LS) MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 60.0 g 58.824 1006.52
2 6.0 g 5.882 23.866
3 1.5 g 1.471 19.726
4 1.0 g 0.98 3.95
5 1.0 g 0.98 18.328
6 0.4 g 0.392 2.667
7 0.4 g 0.392 2.252
8 Trace element solution 10.0 ml - -
9
(BD Bacto)
1.0 g 0.98 -
10
(0.1% w/v)
0.5 ml 4.9e-4 0.002
11 1.5 g 1.471 13.875
12 2.5 g 2.451 22.273
13 Vitamin solution 10.0 ml - -
14 0.5 g 0.49 2.041
15 1000.0 ml 978.634 54322.4
Instructions:
  1. Dissolve ingredients except carbonate, pyruvate, vitamins, and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas ( pyruvate and vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.3 - 7.5, if necessary.
  2. Note: Use at least 5-10%(v/v) inoculum.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 58.833 1006.69
MgSO4 x 7 H2O 5.912 23.985
Na-pyruvate 2.451 22.273
KCl 1.471 19.726
Show more

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

Similar media

284. KOSMOTOGA MEDIUM 90 %
730. FERROGLOBUS PLACIDUS MEDIUM 90 %
731. THERMOSYNTROPHA MEDIUM 90 %
688. THERMOTOGA SUBTERRANEA MEDIUM 90 %
1011c. DESULFOTHERMUS MJ MEDIUM (H2/CO2) 89 %

Latest comments

No comments for this medium.