Loading ...

1526d. GEOHALOVIRGA (L21 LS) MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 60.0 g 59.289 1014.48
2 6.0 g 5.929 29.163
3 1.5 g 1.482 19.882
4 1.0 g 0.988 6.957
5 1.0 g 0.988 18.473
6 0.4 g 0.395 2.689
7 0.4 g 0.395 2.269
8 Trace element solution 10.0 ml - -
9
(0.1% w/v)
0.5 ml 4.9e-4 0.002
10 1.0 g 0.988 9.323
11 1.6 g 1.581 19.273
12 6.4 g 6.324 39.517
13 Seven vitamins solution 1.0 ml - -
14 0.3 g 0.296 1.688
15 0.3 g 0.296 1.234
16 1000.0 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients except carbonate, acetate, fumarate, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, fumarate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (fumarate and vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.9
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: No strains found

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.221 55354.1
NaCl 59.298 1014.65
Na2-fumarate 6.324 39.517
MgCl2 x 6 H2O 5.929 29.163
Sodium acetate 1.581 19.273
Show more

Similar media

814. METHANOBACTERIUM SUBTERRANEUM MEDIUM 88 %
391. METHANOCOCCUS VOLTAE MEDIUM 88 %
1731. PONTIELLA MEDIUM 86 %
1526a. KIRITIMATIELLA (L21 LS) MEDIUM 86 %
1527. SEDIMENTISPHAERA (L21 HS) MEDIUM 86 %

Latest comments

No comments for this medium.