DSMZ 1529 . GEOGLOBUS AHANGARI MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(III) citrate 11.20 g 11.302 46.14
NaCl 19.00 g 19.173 328.072
MgCl2 x 6 H2O 9.00 g 9.082 44.671
MgSO4 x 7 H2O 0.15 g 0.151 0.614
CaCl2 x 2 H2O 0.30 g 0.303 2.059
KCl 0.10 g 0.101 1.354
KH2PO4 0.60 g 0.605 4.449
(NH4)2SO4 0.10 g 0.101 0.764
NaBr 0.05 g 0.05 0.49
SrCl2 0.02 g 0.02 0.127
Trace element solution SL-10 1.00 ml - -
NaHCO3 2.50 g 2.523 30.03
Yeast extract 0.10 g 0.101 -
Na-pyruvate 1.00 g 1.009 9.17
Wolin's vitamin solution 10.00 ml - -
FeCl2 x 4 H2O 0.20 g 0.202 1.015
L-Cysteine HCl x H2O 0.10 g 0.101 0.575
Distilled water 1000.00 ml - -
1 First dissolve ferric citrate in water by heating and adjust to pH 5.8, then cool to room temperature. Add other compounds (except bicarbonate, yeast extract, pyruvate,vitamins, ferrous chloride and cysteine) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then add bicarbonate and adjust pH to 7.2 - 7.5. Dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autocalve. Prior to inoculation add yeast extract, pyruvate, vitamins, ferrous chloride (dissolved in 0.2 N HCl) and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of pyruvate, vitamins and ferrous chloride should be sterilized by filtration. Adjust pH of the complete medium to 6.8 - 7.0.
2 Note: Use 5 - 10% (v/v) inoculum. The color of the medium supernatant changes from orange-brown to yellow-greenish during growth.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 11.05.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.173 328.072
Fe(III) citrate 11.302 46.14
MgCl2 x 6 H2O 9.082 44.671
NaHCO3 2.523 30.03
Na-pyruvate 1.009 9.17
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain