DSMZ 152 . NICOTINIC ACID MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Eubacterium barkeri DSM 1223

This strain uses the standard medium without modifications.


Growth conditions: 35 °C
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nicotinic acid 5.0 g 5 40.614
Yeast extract 10.0 g 10 -
CaCl2 x 2 H2O 8.0 mg 0.008 0.054
MgSO4 x 7 H2O 8.0 mg 0.008 0.032
KH2PO4 40.0 mg 0.04 0.294
K2HPO4 40.0 mg 0.04 0.23
NaCl 80.0 mg 0.08 1.369
NaHCO3 0.4 g 0.4 4.762
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
Distilled water 1000.0 ml - -
1 Add nicotinic acid to the given amount of water, boil until dissolved and cool to room temperature. Add the other substances, adjust pH to 8.2 and distribute under 100% nitrogen atmosphere.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.2
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Associated strains

All strains for this medium: 1
Eubacterium barkeri DSM 1223

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Nicotinic acid 5 40.614
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
NaCl 0.08 1.369
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain