152 .
NICOTINIC ACID MEDIUM
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Selected strain: Eubacterium barkeri DSM 1223
This strain uses the standard medium without modifications.
Growth conditions: 35 °C
Equipment needed:
Final pH:
8.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nicotinic acid | 5.0 | g | ||
|
|
Yeast extract | 10.0 | g | ||
|
|
CaCl2 x 2 H2O | 8.0 | mg | ||
|
|
MgSO4 x 7 H2O | 8.0 | mg | ||
|
|
KH2PO4 | 40.0 | mg | ||
|
|
K2HPO4 | 40.0 | mg | ||
|
|
NaCl | 80.0 | mg | ||
|
|
NaHCO3 | 0.4 | g | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Add nicotinic acid to the given amount of water, boil until dissolved and cool to room temperature. Add the other substances, adjust pH to 8.2 and distribute under 100% nitrogen atmosphere. | |||||